FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475936
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT5910.12417635984670208Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG5720.1201842264506152Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA5340.11219995965844147Illumina Single End PCR Primer 1 (96% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC5000.10505614200228602Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA4760.1000134471861763Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGTC556.002665E-1140.9090921
GTGTTTC3250.040.846151
GTGTCTT403.4525328E-739.3750041
GGGGCTA356.240578E-638.5714261
CGCGGTC301.13891685E-437.5000044
GAGTCTT1150.037.1739161
GGGTGTC250.002105870536.01
GTGGGTC250.002105870536.01
ACATTCG250.002105870536.05
ACCCTGT250.002105870536.01
AGTCTGG250.002105870536.045
GGTGCAC250.002105870536.01
TTGTTTC1000.036.01
GTGGTTC250.002105870536.01
TCGCGCT401.5586531E-533.7500041
GACTCTT4850.032.4742281
TGTTACC352.817911E-432.1428572
TGTGTCC708.112693E-1032.1428572
TCACTCT22350.031.7114091
ACACTCT48100.030.1715181
Produced by FastQC (version 0.11.5)