FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073840
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC16210.1509535871265738Illumina Single End PCR Primer 1 (96% over 26bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT15620.14545928629963495Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG14080.1311182299038963Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC13550.12618267153393428Illumina Single End PCR Primer 1 (96% over 31bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA13530.12599642404827535Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT12640.11770841093645236Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA12590.117242792222305Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT12560.1169634209938166Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA11650.10848916039633465Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT11630.1083029129106757Illumina Single End PCR Primer 1 (100% over 30bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA11510.10718542799672205Illumina Single End PCR Primer 1 (96% over 27bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG11440.10653356179691573Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA11240.10467108694032631Illumina Single End PCR Primer 1 (96% over 28bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT11110.10346047828354316Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGG207.0332957E-444.999724
GTCTTCG207.0332957E-444.9997211
CGTATGG451.9281288E-839.99975245
CCCGGGT403.450532E-739.3857571
TGGTCGG356.236198E-638.5819661
CCGGCTT301.1399134E-437.4997672
GTTTGGC552.741217E-936.8282431
GTGTTTC5350.036.598781
GGGTCCA250.002104223536.009841
CGGGCAG250.002107111335.999782
TTTGGCG250.002107111335.999782
CGCGTTG250.002107111335.999783
GGCCTCT250.002107111335.999786
GCTTTAC250.002107111335.999785
GGGGCTT250.002107111335.999782
GTGTGTC1400.035.3668021
GTGGTTC702.1827873E-1135.3668021
GAGTCTT1150.035.2270161
TGCCCGC458.699644E-734.9997832
GCGCTTT653.6743586E-1034.615173
Produced by FastQC (version 0.11.5)