FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909864
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC15510.17046503653293238Illumina Single End PCR Primer 1 (96% over 31bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT14000.15386914967511628Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA11860.13034915108191994Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT11770.1293599922625799Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG11580.12727176808841761Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG11170.12276560013364635Illumina Single End PCR Primer 1 (96% over 30bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT10800.118699058320804Illumina Single End PCR Primer 1 (96% over 32bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA10360.11386317075958606Illumina Single End PCR Primer 1 (96% over 28bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC10250.11265419886928156Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA10190.11199475965638821Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC10070.1106758812306015Illumina Single End PCR Primer 1 (96% over 26bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT9910.10891737666288588Illumina Single End PCR Primer 1 (100% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT9770.1073786851661347Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG9440.10375176949522127Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA9110.1001248538243078Illumina Single End PCR Primer 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTTT253.8901904E-544.9999473
GTGTGTC1250.039.6021271
GGGTGTC356.244549E-638.57351
CAAGCTT356.246906E-638.5713842
TCGCGCG301.13937436E-437.5020141
CGTATGG301.1397431E-437.49995845
GTTTCGA301.1397431E-437.4999586
GCGCTTT601.546141E-1037.4999583
GTGTTTC5050.036.982181
GGGTTCC250.002106890835.9999582
GCCCGCT401.559637E-533.7518121
GGTGTCC401.560224E-533.7499582
TCACTCT43850.033.0462461
GTGTCCC754.7293724E-1132.999963
ATGGCGT352.8199385E-432.1428241
CGGTTTG352.8199385E-432.142823
TCTTAGT352.8199385E-432.1428222
AGGGGGG352.8199385E-432.1428241
GCGTGGG352.8199385E-432.142822
GACTCTT9550.032.0436061
Produced by FastQC (version 0.11.5)