FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539022
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT8280.15361154090185558Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC7510.13932640968272167Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA6990.12967930808018968Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG6940.1287517021568693Illumina Single End PCR Primer 1 (96% over 29bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT6340.11762043107702469Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA5510.10222217274990632Illumina Single End PCR Primer 1 (96% over 27bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC5480.10166560919591407Illumina Single End PCR Primer 1 (96% over 26bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT5430.10073800327259369Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCGG207.02995E-445.09
GTGTCTT207.02995E-445.01
GTTTTTC253.88804E-545.01
GTGCCCG207.02995E-445.01
GCCGGGT603.6379788E-1241.2499961
TTGTTTC451.9250365E-840.0000041
GTGTTTC3550.039.9295771
TCTGTGG403.4536606E-739.3753
CGCGGAG356.242117E-638.5714262
GTGTGTC659.094947E-1238.0769271
CCCGCTT659.094947E-1238.0769272
TGTGTCA301.1391178E-437.4999962
CCCGCTG301.1391178E-437.4999962
GTGTTGC250.002106118236.01
GAGTCTT1000.036.01
TATGGCT250.002106118236.05
TTGTCTT250.002106118236.01
TGGGGGA250.002106118236.01
CATCCTT250.002106118236.022
GGGGCGT250.002106118236.04
Produced by FastQC (version 0.11.5)