FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559820
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGT253.8862272E-545.0039331
GCCGGGT403.4516233E-739.3784371
AAACTTT403.4540426E-739.374923
AGGGTTT356.2426334E-638.5713543
CCGGCTT301.13918744E-437.4999242
CGTTCCA301.13918744E-437.4999243
GTATCCC301.13918744E-437.4999243
CCGGGTT800.036.5624242
GGCTTGG250.002106207735.9999344
GTGTTTC3650.033.907071
GTTACAC551.1250813E-732.7301331
CACGCTT1800.032.4999352
CAGGGTT352.818574E-432.1427962
GTGTGTC1000.031.5027521
CCCGCTT501.9792496E-631.4999392
GTCTTTC17150.031.489631
TCACTCT22450.030.3701381
ACACTCT53300.030.3544351
GACTCTT6850.030.2216191
TCAGGGT300.005142833630.002621
Produced by FastQC (version 0.11.5)