FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757707
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT9400.12405850810405605Illumina Single End PCR Primer 1 (96% over 30bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA7600.10030262357349214Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGT207.0276484E-445.005351
GTGTGGC403.4529148E-739.379681
TTTGGGG301.1387578E-437.504461
GGGGTTC552.743036E-936.8225561
GTGTTTC4350.036.2111971
GGGGCTC250.002105428436.0042761
TTAATCG250.002106792835.99952330
CCGGGTT401.5600086E-533.7495542
GCCGGTC401.5600086E-533.7495547
CCCGCTT803.6379788E-1233.7495542
GACTCTT9400.033.0358351
GGGTTCC551.1269731E-732.726842
GTATCCG352.819694E-432.14243345
CGGGTTG501.9806448E-631.4995843
TATGGGG501.9806448E-631.49958445
GTGTGTC801.0004442E-1030.9411771
ACACTCT76050.030.1219231
GGGTACA453.4864614E-530.0035651
GACTGTT453.4864614E-530.0035651
AATCATT453.4896046E-529.9996033
Produced by FastQC (version 0.11.5)