FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_2MG_N-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_2MG_N-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177009
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTT253.8647588E-545.024871
CCCGCTT207.0175383E-444.9994352
TGGCGGA301.1323328E-437.5207251
GCCCTCT301.1323328E-437.5207251
CTACCAA250.002102435835.9995523
GACTCTT1900.034.3610881
TGTCACA352.8110167E-432.14245235
GTGTTTC751.6734703E-930.0165821
GGGTCTT300.005121774530.016581
CCTTTCC300.005121774530.016581
CGTATGC300.00513595429.99962245
TACATAG300.00513595429.99962232
TCACTCT5200.029.4393391
ACACTCT12800.028.8440591
GTCTTTC4500.028.5157531
GTTTCCC4200.027.8725411
GCTTTCG507.1254246E-526.999665
TGGGGGG608.105611E-626.2645071
CGAGGTC450.001222547624.99968744
CACGCTT551.3619427E-424.5451472
Produced by FastQC (version 0.11.5)