FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7151325
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA172490.24120005733203287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG343050.090.184476
GGAAACG384450.088.280475
GGGAAAC445550.085.213654
AAACGGA365100.084.982047
GGGGAAA553050.081.669073
GAATCGG50050.080.2800756
GGGGGAA667700.079.088082
GGTATCG35450.078.643595
GGAATCG51900.077.5099645
GTATCGG37250.074.0783546
AACGGAA462850.074.053028
GGGGGGA849850.065.2081
ACGGAAC82650.064.8179869
ACGGAAA219750.064.015569
GGGAATC66200.062.632214
AATCGGA64650.062.4419827
GAAACCG54600.062.0209436
AACCGAA75050.062.01578
ACCGAAA61850.058.5119559
GGTAACG29850.057.5977755
Produced by FastQC (version 0.11.5)