FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12312458
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC783190.6360955708437747TruSeq Adapter, Index 22 (97% over 34bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC629600.5113519981144301TruSeq Adapter, Index 11 (97% over 35bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACGGTAGCATCTC432810.351522011283206TruSeq Adapter, Index 11 (96% over 32bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC432100.35094535956995754TruSeq Adapter, Index 11 (97% over 35bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC368210.29905482723271015TruSeq Adapter, Index 11 (96% over 32bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGGTAGCATCTC324770.26377348860804234Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC272350.22119872408904867TruSeq Adapter, Index 11 (96% over 32bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACGGTAGCATCTC190610.15481068036942747Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAACAGCACACGTCTGAACTCCAGTCACGGTAGCATCTC156230.12688774248001494TruSeq Adapter, Index 11 (96% over 32bp)
GGGGGGAAACGGAACAGCACACGTCTGAACACCAGTCACGGTAGCATCTC144560.11740953755943777Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGGTAGCAACTC124270.100930293528717Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG1800700.093.388826
GGAAACG1986550.091.583695
AAACGGA1854850.090.7663357
GAATCGG233750.090.38776
GGGAAAC2265850.090.210394
GGGGAAA2670450.088.746533
GGAATCG239700.088.560185
GGTATCG135600.088.344915
GTATCGG136550.087.382436
GGGGGAA3105850.086.074222
AACGGAA2179050.084.7803348
ACGGAAA972350.084.716399
GAAACCG246350.084.4535146
AACCGAA336800.083.406548
GGGGGGA3298850.082.1432341
AAACCGA256000.081.547347
ACCGAAA273900.081.455319
AATCGGA258450.081.4543157
GGGAATC267350.080.538164
GGTAACG99550.079.874915
Produced by FastQC (version 0.11.5)