FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11194703
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC677210.6049378889283619TruSeq Adapter, Index 7 (96% over 33bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC591250.5281515731145346TruSeq Adapter, Index 9 (97% over 35bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC418510.37384645220154566TruSeq Adapter, Index 9 (97% over 35bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACGAGTGGATCTC377270.3370076008269268TruSeq Adapter, Index 9 (96% over 32bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC229480.20498980633965902TruSeq Adapter, Index 9 (96% over 32bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGAGTGGATCTC196930.17591355483035145Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC187630.1676060543991207TruSeq Adapter, Index 9 (96% over 32bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACGAGTGGATCTC130130.11624247646409198Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAAGAGCACACGTCTTAACTCCAGTCACGAGTGGATCTC124950.11161528805185811TruSeq Adapter, Index 9 (96% over 32bp)
GGGGGGAAACGGAACAGCACACGTCTGAACTCCAGTCACGAGTGGATCTC121810.1088103900567974TruSeq Adapter, Index 9 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG1923450.093.838596
AAACGGA1956200.092.391417
GGAAACG2130300.091.610095
GAATCGG261250.091.490196
GGGAAAC2424050.090.531564
GGAATCG265200.090.342435
GTATCGG154950.089.9412166
AACGGAA2209200.089.729418
GGGGAAA2813600.089.50753
GGTATCG156150.089.402125
ACGGAAA925900.087.719649
AACCGAA351350.087.13118
ACGGAAC359850.086.986959
GGGGGAA3266050.086.609512
AATCGGA276350.086.198897
GAAACGT124450.084.8466646
ACCGAAA274550.084.7571269
AAACCGA268900.084.50667
GGGAATC288100.084.0682454
ACGGAAG646050.083.52239
Produced by FastQC (version 0.11.5)