FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11598823
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTC571090.4923689239847871TruSeq Adapter, Index 22 (97% over 41bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTC447420.38574603647283867TruSeq Adapter, Index 22 (97% over 39bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACCGTACGATCTC324600.2798559819388571TruSeq Adapter, Index 22 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACCGTACGATCTC263200.22691957623631295TruSeq Adapter, Index 22 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACCGTACGATCTC238120.20529669260406855Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACCGTACGATCTC196570.16947409232816124TruSeq Adapter, Index 22 (97% over 36bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACCGTACGATCTC141880.12232275636933161Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAACAGCACACGTCTGAACTCCAGTCACCGTACGATCTC116620.10054468457704718TruSeq Adapter, Index 22 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG1227900.092.856086
GGAAACG1369500.090.774665
AAACGGA1264900.090.301397
GAATCGG161000.090.042686
GGGAAAC1579900.088.652464
GGTATCG99250.087.006785
GGAATCG167950.086.995365
GGGGAAA1882300.086.583333
GTATCGG99600.086.4148946
AACGGAA1491100.084.298938
GGGGGAA2216850.083.984882
ACGGAAA665650.080.6704259
ACGGAAC242700.079.987959
GGGGGGA2389650.079.353651
AACCGAA242750.078.738758
GAAACGT92550.077.6519246
ACCGAAA193900.077.483849
GAAACCG184950.077.2526256
AATCGGA190150.076.039297
AAACCGA188200.076.0195167
Produced by FastQC (version 0.11.5)