FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9765345
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC617730.6325736571518978TruSeq Adapter, Index 21 (97% over 41bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC470790.4821027828509899TruSeq Adapter, Index 21 (97% over 39bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACGTTTCGATCTC343830.3520920151822593TruSeq Adapter, Index 21 (97% over 36bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC318530.3261840723497224TruSeq Adapter, Index 21 (97% over 39bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC269920.27640600511297864TruSeq Adapter, Index 21 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGTTTCGATCTC240190.24596161221134535Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC211040.21611115633907454TruSeq Adapter, Index 21 (97% over 36bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACGTTTCGATCTC148130.15168946924046206Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAACAGCACACGTCTGAACTCCAGTCACGTTTCGATCTC118390.12123483604521909TruSeq Adapter, Index 21 (97% over 36bp)
GGGGGGAAACGGAACAGCACACGTCTGAACACCAGTCACGTTTCGATCTC108800.11141439447351835Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG1318550.093.5725866
AAACGGA1343050.092.021257
GGAAACG1475950.091.4333345
GAATCGG178200.090.281176
GGGAAAC1682700.090.050744
AACGGAA1533600.089.293988
GGAATCG181250.089.286095
GGGGAAA1983300.088.269263
GGTATCG104400.087.9475
GTATCGG104900.087.437246
GGGGGAA2313400.085.380432
ACGGAAA680250.085.0558249
AACCGAA251150.084.672738
ACGGAAC233450.084.3577659
AATCGGA190650.084.285887
ACGGAAG431550.082.561539
ACCGAAA201450.082.0071959
GAAACCG186900.081.809056
AAACCGA188450.081.3630147
GGTAACG81050.081.109695
Produced by FastQC (version 0.11.5)