FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8228775
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC380260.4621100953665642TruSeq Adapter, Index 20 (97% over 41bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC317580.3859383687122324TruSeq Adapter, Index 20 (97% over 39bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC218820.2659205045708505TruSeq Adapter, Index 20 (97% over 39bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACGTGGCCATCTC207880.252625694590021TruSeq Adapter, Index 20 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC171700.2086580323316654TruSeq Adapter, Index 20 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGTGGCCATCTC145380.17667271252403913Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACGTGGCCATCTC121220.14731232777661318TruSeq Adapter, Index 20 (97% over 36bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACGTGGCCATCTC85460.10385506955774097Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG971000.093.365846
AAACGGA994750.091.040647
GGAAACG1071150.090.822445
GAATCGG130250.089.237746
GGGAAAC1222250.088.999334
GGAATCG133000.087.892595
GGTATCG77000.087.473765
GGGGAAA1444800.087.053483
GTATCGG78000.086.2914056
AACGGAA1154800.086.0939948
GGGGGAA1680750.084.6515352
ACGGAAC194700.082.3130659
AACCGAA177400.082.173728
GAAACCG129750.081.783966
ACGGAAA535200.081.349239
AATCGGA142500.081.0992667
GGGGGGA1823200.079.345991
AAACCGA135150.078.832067
GGGAATC151200.078.443884
ACCGAAA145700.077.851049
Produced by FastQC (version 0.11.5)