FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9087608
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC283690.31217235602592014TruSeq Adapter, Index 3 (97% over 34bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC228810.25178242723497757TruSeq Adapter, Index 18 (97% over 35bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACGTAGAGATCTC148280.16316724929156276TruSeq Adapter, Index 18 (96% over 32bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC136570.1502815702437869TruSeq Adapter, Index 18 (97% over 35bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC118470.13036433789837765TruSeq Adapter, Index 18 (96% over 32bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACGTAGAGATCTC98720.10863144625076258Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACGTAGAGATCTC94590.10408679599736255TruSeq Adapter, Index 18 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG917050.092.518416
GGAAACG1023500.090.725175
AAACGGA948100.089.7384647
GAATCGG126800.088.893416
GGGAAAC1194900.088.8855744
GGAATCG129250.087.649395
GGTATCG70550.087.189435
GTATCGG70750.086.540146
GGGGAAA1509700.086.095413
AACGGAA1114200.083.7315448
GGGGGAA1838000.083.427382
AACCGAA185500.080.096128
GAAACCG140450.079.882056
ACGGAAC165850.078.73149
GGGGGGA2020400.077.958111
AATCGGA145300.077.4767467
GGTAACG52200.077.16435
AAACCGA145850.076.989187
ACCGAAA154700.076.269339
GGGAATC155100.076.2568054
Produced by FastQC (version 0.11.5)