FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9279323
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAAACGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTC436630.47054079268498356TruSeq Adapter, Index 16 (97% over 41bp)
GGGGGGAATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTC344670.3714387353473955TruSeq Adapter, Index 16 (97% over 39bp)
GGGGGGAAACGGAAGAGCACACGTCTGAACACCAGTCACCCGTCCATCTC251780.27133444972224807TruSeq Adapter, Index 16 (97% over 36bp)
GGGGGGTATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTC239600.2582084921496967TruSeq Adapter, Index 16 (97% over 39bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACTCCAGTCACCCGTCCATCTC188440.20307515968567966TruSeq Adapter, Index 16 (97% over 36bp)
GGGGGGAAACGGAAAAGCACACGTCTGAACACCAGTCACCCGTCCATCTC166510.17944197006613521Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGGGGAAACGGAATAGCACACGTCTGAACTCCAGTCACCCGTCCATCTC136100.1466701827277701TruSeq Adapter, Index 16 (97% over 36bp)
GGGGGGAAACGGAATAGCACACGTCTGAACACCAGTCACCCGTCCATCTC101390.10926443664047475Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG940300.092.969286
GGAAACG1049550.090.677925
AAACGGA971600.090.173757
GGGAAAC1209450.088.57874
GAATCGG127800.087.232026
GGTATCG78000.086.717965
GGGGAAA1432900.086.692733
GGAATCG131200.085.4058765
AACGGAA1138350.084.642448
GTATCGG79400.084.471046
GGGGGAA1687200.083.810832
AACCGAA179800.080.3898548
GAAACCG135850.080.314696
ACGGAAA509750.079.978059
ACGGAAC199800.079.023189
GGGGGGA1839650.078.218561
AATCGGA144650.077.004027
ACCGAAA144250.076.7565469
GGTAACG57400.075.553155
AAACCGA145400.075.006077
Produced by FastQC (version 0.11.5)