FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13092274
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACGG180050.073.841386
GGAAACG198100.073.539265
GGGGGAA866100.068.240212
GGGGAAA603800.063.8391463
GGGAAAC300850.063.769624
GGGGGGA1084250.060.3289871
CCCCCCT2058300.058.9404452
GAATCGG30500.058.4011546
GGGGGTA380300.056.2587742
AAACGGA244200.054.4241947
CCCCCCC7336300.053.7074621
CCCCCTT1203900.053.694213
CCCCCTA345200.051.572773
GGGGTAA203700.051.184113
TTTTTTG1558850.049.8292962
CCCCCCA2255750.049.8159642
GGAATCG36250.049.6616555
GGGGGTT881800.049.3181152
CCCCCAT826350.046.6289443
CCCCTTT689000.046.527554
Produced by FastQC (version 0.11.5)