FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601378
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC385400.070.499063
CCCCCCT1294750.066.424842
AAAAAAG255350.062.189692
CCCCCTA173100.059.4374853
CCCCCTT570650.058.8835873
CCCCTCA121150.057.8319244
CCCCTCC222700.057.674724
AAAAAGC116500.057.2861253
GGGGGTT406500.055.320442
CCCCTTT269000.054.652174
CCCCTAT56000.053.94714
CCCCCCC4447600.052.9278641
CCCCTCT90850.052.4937744
TTTTTTG249750.051.2210852
GGGGTTC125050.051.0522163
AAAAATC119850.050.7703443
CCCCTTA136950.049.425594
CCCCTCG52300.048.7721184
CCCCTAA80700.048.148064
AAAAAAT410700.047.7339822
Produced by FastQC (version 0.11.5)