FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4769858
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC382950.070.577293
CCCCCCT1302550.065.647062
AAAAAAG260200.060.8472632
CCCCTCC216900.059.325894
CCCCCTT571850.057.704543
CCCCTCA120400.057.520924
CCCCCTA179800.057.4069983
AAAAAGC119850.056.7544333
GGGGGTT428500.054.508272
CCCCTTT268150.053.992324
CCCCTCT85950.053.1095124
CCCCCCC4575900.052.8410381
GGGGTTC129900.051.778433
CCCCTAT59250.051.3081634
TTTTTTG292700.050.3908772
CCCCTCG53500.049.8973354
AAAAATC122400.048.4703143
CCCCCTG321850.047.153313
CCCCTTA139800.046.8885424
CCCCCCG554600.046.7055662
Produced by FastQC (version 0.11.5)