FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691253
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC406400.072.173293
CCCCCCT1384850.066.28512
CCCCTCA126900.062.39754
CCCCTCC225150.061.3501554
AAAAAAG265900.060.2082062
CCCCCTT612450.058.9667973
CCCCCTA184750.057.3598943
AAAAAGC119950.056.1921123
CCCCTCT91850.054.3004384
CCCCTTT291750.054.215894
GGGGGTT400300.052.774692
CCCCTAT58000.052.2499544
CCCCCCC4774400.052.2253951
GGGGTTC128650.051.5059783
CCCCTCG52450.049.8092964
CCCCTTA150200.049.1444324
AAAAATC123200.047.8469543
CCCCTAA82450.047.7592054
CCCCCTG334600.047.0882723
GGGGGTC126350.046.654093
Produced by FastQC (version 0.11.5)