FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5045544
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC413600.071.2956853
CCCCCCT1409750.065.879972
CCCCTCA127200.059.8975834
AAAAAAG280000.059.481292
CCCCTCC240450.059.4019554
CCCCCTA186600.058.9547733
CCCCCTT623100.057.92833
AAAAAGC126650.057.2323273
CCCCTAT62650.054.8162464
GGGGGTT424100.054.2802282
CCCCTTT299000.053.4095084
CCCCTCT92050.053.0470664
CCCCCCC5027700.052.3842051
GGGGTTC135900.051.5892223
CCCCTCG53350.050.838624
AAAAATC134400.050.0091253
TTTTTTG297150.049.573842
CCCCTTA151350.048.3942764
CCCCCTG342850.047.506763
CCCCCGC184950.047.2301643
Produced by FastQC (version 0.11.5)