FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909350
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC320750.072.875453
CCCCCCT1104400.066.204182
CCCCTCC181150.061.830114
AAAAAAG213500.060.763192
CCCCTCA100300.059.1501774
CCCCCTT492150.058.517353
AAAAAGC101600.058.3465843
CCCCCTA146300.058.0196043
CCCCTCT71250.055.6667864
CCCCTTT235700.054.734954
GGGGGTT330450.054.1653942
CCCCTAT48300.052.6139874
CCCCCCC3908350.052.4838941
AAAAATC101400.052.0443
CCCCTCG44000.051.710344
GGGGTTC111500.050.524773
TTTTTTG220550.049.5594332
CCCCTTA116600.048.762974
CCCCTAA68200.047.0125664
CCCCCCG477050.046.4619672
Produced by FastQC (version 0.11.5)