FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4761736
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC385450.071.006133
CCCCCCT1313100.064.436642
AAAAAAG249500.063.1909262
CCCCTCC220400.060.042694
AAAAAGC120150.058.3121263
CCCCTCA119000.057.1193584
CCCCCTT584900.056.2378583
CCCCCTA166000.055.082453
CCCCTCT87050.053.8566134
GGGGGTT381900.053.6224142
CCCCTTT275700.052.9439624
GGGGTTC126500.051.9304733
AAAAATC122850.051.81083
TTTTTTG292200.051.6644332
CCCCCCC4815950.051.6617551
CCCCTCG53650.049.491744
CCCCTAT54550.048.7622684
CCCCCCG608600.046.8470272
CCCCCGC184300.046.674943
AAAATCG18450.046.59864
Produced by FastQC (version 0.11.5)