FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4968070
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC403750.071.740213
CCCCCCT1385850.065.827552
AAAAAAG269200.060.665312
CCCCTCC233800.059.6078384
CCCCTCA126050.058.78544
CCCCCTT614550.057.643843
AAAAAGC123700.057.099073
CCCCCTA181450.056.412853
CCCCTCT89050.054.0335274
CCCCTTT298500.053.5780074
GGGGGTT422450.053.286972
CCCCCCC4918300.052.286411
CCCCTAT61400.051.908914
AAAAATC126700.051.885653
CCCCTCG55250.050.8092734
GGGGTTC133800.050.019883
CCCCCTG341200.048.6548233
TTTTTTG290550.047.2810442
CCCCTTA145650.046.8960384
CCCCCCG594650.046.243612
Produced by FastQC (version 0.11.5)