FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5488635
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC461950.072.0282753
CCCCCCT1586850.066.383622
AAAAAAG277100.061.0277062
CCCCTCC268150.059.9612544
CCCCCTT704250.058.7126663
CCCCCTA209150.058.4121173
CCCCTCA136350.058.2466554
AAAAAGC127750.058.0405963
CCCCTCT104450.055.5715644
CCCCTTT333550.054.370694
CCCCTCG64300.053.6309554
GGGGGTT452650.053.289762
CCCCTAT66250.052.8410534
CCCCCCC5599800.052.2734071
GGGGTTC151950.050.3598823
TTTTTTG310950.049.5110442
CCCCTTA166250.049.1424334
AAAAATC139700.048.8936353
CCCCTAA93550.048.235824
CCCCCTG387100.047.781813
Produced by FastQC (version 0.11.5)