FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4954092
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC340250.068.893483
AAAAAAG295500.065.2494662
CCCCCCT1191750.063.2647632
AAAAAGC129650.060.707043
CCCCCTA162100.055.6163253
GGGGGTT493750.055.3577162
CCCCCTT548800.055.142013
CCCCTCC200650.054.5658384
CCCCTCA109050.054.4033784
GGGGTTC155500.054.005873
AAAAATC127000.052.4738663
CCCCCCC4351450.051.9944761
CCCCTCT85950.051.3956264
CCCCTTT264150.051.3206834
TTTTTTG275800.050.2409132
AAAAAAT443950.049.0378232
CCCCTAT55550.048.3973354
AAAAAGG138100.048.3249863
CCCCTAA72900.046.6524624
CCCCCCG545800.045.7008062
Produced by FastQC (version 0.11.5)