FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4254871
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC314750.068.8166963
AAAAAAG240150.064.858792
CCCCCCT1077350.062.900692
AAAAAGC110650.060.829343
CCCCTCA95650.056.066534
CCCCTCC186850.055.9527744
GGGGGTT402500.055.4561272
CCCCCTA143850.054.6490753
CCCCCTT493000.054.5817763
GGGGTTC130100.053.195753
AAAAATC116300.052.727993
CCCCTCT73000.052.0549244
CCCCTAT45500.051.571564
CCCCTTT231800.051.3525854
CCCCCCC3906100.051.3519441
TTTTTTG226700.049.073312
AAAAAAT373150.048.997562
AAAAAGG117050.046.952273
GGGGGTC126800.046.488683
GGGGTTT184950.045.2528843
Produced by FastQC (version 0.11.5)