FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639094
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC351200.071.6281363
CCCCCCT1228700.065.309942
AAAAAAG267400.062.4072532
AAAAAGC120100.060.3533973
CCCCTCC195900.059.9382744
CCCCCTA160100.058.15073
CCCCTCA112250.057.5496564
CCCCCTT558650.057.3073463
GGGGGTT449250.055.8401262
CCCCTCT82400.055.0511474
CCCCTTT270750.054.2802774
CCCCTAT52750.053.3076444
GGGGTTC143800.053.214123
CCCCCCC4279550.052.681941
CCCCTCG47350.051.1611634
AAAAATC119750.050.534053
AAAAAAT419300.047.933212
TTTTTTG249550.046.9982642
CCCCTAA72000.045.454514
CCCCCTG303600.045.3718453
Produced by FastQC (version 0.11.5)