FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4906691
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC353300.069.777743
AAAAAAG297850.064.2076952
CCCCCCT1264350.063.4560782
AAAAAGC134150.058.4941673
CCCCTCC200700.056.6828044
CCCCCTA171600.056.6622433
GGGGGTT499300.055.8263782
CCCCCTT575450.055.6841933
CCCCTCA112600.055.6837844
GGGGTTC157950.054.552023
CCCCTCT87500.053.7428054
AAAAATC136650.052.766143
CCCCTTT270650.052.5983244
CCCCCCC4462000.052.062051
GGGGGTC149250.049.680023
AAAAAGG138100.049.598073
CCCCTAT60700.049.456294
AAAAAAT456200.048.6472662
TTTTTTG253300.045.570312
GGGGTTT232600.045.1106643
Produced by FastQC (version 0.11.5)