FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059558
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC317000.071.745443
CCCCCCT1094600.065.369752
AAAAAAG251700.064.733892
AAAAAGC112800.059.7126773
CCCCTCA98350.059.5991634
CCCCTCC178750.059.2595334
CCCCTCT73550.058.9640774
CCCCCTT522250.057.6010553
CCCCCTA142400.057.0741463
CCCCTTT257150.054.270614
GGGGGTT364600.054.251892
CCCCCCC3778100.052.523471
GGGGTTC117500.051.78583
AAAAATC115650.051.2998623
CCCCTAT48500.051.1243974
AAAAAGG117100.049.4477163
CCCCTCG39900.048.9292224
AAAAAAT403300.046.961162
TTTTTTG249600.046.779772
GGGGGTC105350.046.5312353
Produced by FastQC (version 0.11.5)