FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5096024
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC373550.071.5398253
CCCCCCT1334400.064.792272
AAAAAAG288850.063.643282
CCCCTCC211850.059.910774
AAAAAGC134350.059.7864653
CCCCCTA176750.057.6454163
CCCCCTT616100.056.6288223
CCCCTCA116800.056.5286874
GGGGGTT489100.056.223752
CCCCTCT86350.055.6692164
GGGGTTC155850.055.5313843
AAAAATC137050.053.5483863
CCCCTTT296100.053.227364
CCCCCCC4696200.052.630551
CCCCTAT59600.050.4492154
AAAAAAT463450.050.1620252
CCCCTCG52600.050.02894
GGGGGTC150250.048.338143
CCCCTAA78700.047.077874
TTTTTTG278400.046.92212
Produced by FastQC (version 0.11.5)