FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4619449
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC302400.070.212823
AAAAAAG274150.064.666852
CCCCCCT1098000.063.0874632
AAAAAGC123250.062.5878143
CCCCTCA98950.056.4043734
CCCCTCC175350.055.910664
GGGGGTT456950.055.3266642
CCCCCTT517250.055.2548263
AAAAATC120400.054.2064443
CCCCCTA147700.054.0602343
GGGGTTC139550.053.9498333
CCCCTTT250350.052.1955764
CCCCCCC3960150.052.1519971
CCCCTCT74350.051.93984
CCCCTAT52150.050.004464
AAAAAAT417750.048.180392
TTTTTTG279350.047.682352
GGGGGTC141200.047.4324043
CCCCTCG42250.046.9937674
AAAAAGG130400.046.2247663
Produced by FastQC (version 0.11.5)