FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5571297
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC448500.071.858543
CCCCCCT1546750.065.291492
AAAAAAG281250.063.17032
CCCCTCC263350.060.062294
AAAAAGC139250.059.6262133
CCCCTCA131350.058.402774
CCCCCTT698900.056.980633
CCCCTCT96400.056.270414
CCCCCTA193250.056.2376943
GGGGGTT454000.054.4103282
CCCCTTT328800.053.35054
TTTTTTG361000.052.8206832
GGGGTTC148300.052.8166053
CCCCCCC5503850.052.2424551
CCCCTCG63700.052.1229174
AAAAATC140550.050.1526683
CCCCCGC214700.048.3404123
CCCCCTG381500.047.4125563
CCCCTAA84000.047.3300674
CCCCTAT62200.047.194254
Produced by FastQC (version 0.11.5)