FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658639
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC265950.068.261863
AAAAAAG229350.063.4782752
CCCCCCT879100.061.0243072
AAAAAGC107850.060.1614043
AAAAATC117050.056.85313
GGGGTTC127300.056.4171073
GGGGGTT397050.055.7127232
CCCCTCC160700.055.4208034
CCCCCTA120100.054.974243
CCCCTCT69000.052.5246164
CCCCCTT385150.051.8465923
CCCCTCA78500.051.553414
GGGGGTC118900.050.1759033
CCCCCCC3295650.049.6748431
CCCCTAT42850.049.5500534
AAAAAAT359350.049.54222
AAAAAGG108400.047.67463
CCCCTTT181150.046.8569154
GGGTTCC83850.046.281394
TTTTTTG185800.045.42922
Produced by FastQC (version 0.11.5)