FastQCFastQC Report
Wed 29 Jul 2020
HKMNVDRXX_n01_mb285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMNVDRXX_n01_mb285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4885380
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTC396000.070.854013
CCCCCCT1339400.063.248652
AAAAAAG280150.061.8248632
CCCCTCC229200.059.2505234
AAAAAGC137650.058.9047053
CCCCTCA120400.056.6527024
CCCCTCT93150.055.9902154
CCCCCTA176700.055.0536423
CCCCCTT576600.055.012893
GGGGGTT460450.054.9588132
GGGGTTC154650.053.074573
GGGGGTC140100.052.958493
AAAAATC142350.052.7554173
CCCCTTT273900.051.3845184
CCCCCCC4860650.050.397181
CCCCTAT60150.047.697324
CCCCTCG51900.047.2253344
AAAAAAT466900.047.057082
TTTTTTG253050.046.838322
CCCCCTG344900.044.125743
Produced by FastQC (version 0.11.5)