FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114428678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5657810.49443986410469587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTGT930050.085.4708943
TGTGGGG928450.084.795147
GTGTGGG936750.084.334186
GGGTGTG943900.084.1764454
TATACGG73600.078.644957
GTATAGC55800.076.16256
GGTATAC57450.075.383025
CGTATAG65100.073.673135
GCTATAC56900.073.5245365
GTATACG63200.073.480846
CTATAGG69350.073.263846
TATAAGG88150.072.93597
TATACTG85450.072.517597
CCGTATA48450.072.13614
CTATACG52800.071.856366
TATAGGG113900.070.787887
GTATAGG111950.070.664826
TTATACG75650.070.238226
CGCTATA58750.069.835234
GGTATAG105600.069.565695
Produced by FastQC (version 0.11.5)