FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5976290
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG195050.326373050839233No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA137900.23074516129572026No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA74090.12397323423060126No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA69860.11689526445336489No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT68510.11463633792871497No Hit
TGAAAATAAGCGTAGATCCGGAGATTCCCGAATAGGTTAACCTTTTGAAC61340.10263892816446325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG68300.092.4941566
GTATACC58650.091.2723546
GTATACG87650.091.041786
CGTATAC58350.091.011285
GTATAGG152700.090.860666
GGTATAG132850.090.78115
GCTATAC62050.090.1774145
TTATACG98500.090.030876
GTATAGC79600.089.388596
CGTATAG88450.089.361365
TATAGGC115650.089.0424357
CCTATAC45150.088.5826345
GGTATAC78450.088.5214845
AGTATAG88700.088.5204245
AGTATAC52050.088.429535
GTTATAG136500.088.179625
TTATAGC107650.087.9386
CCTATAG51600.087.819895
TATACGC70900.087.62847
GTATAGT112550.087.612326
Produced by FastQC (version 0.11.5)