FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4627614
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG103000.090.111775
CTATACG47750.089.527956
CTATAGG61200.088.3243266
CGTATAC44900.087.594775
GTATAGC61650.087.140296
GTATAGG109000.086.893686
TATAGGC69200.086.212137
TATACCG55700.085.958767
GGTATAA96700.085.863615
CCTATAC33750.085.851975
TATAGGG119250.085.717347
CGTATAG58150.085.606315
CCTATAG38050.085.51265
TATACGG86800.085.312217
TTATACG72850.084.958026
AGTATAG75000.084.803455
TATAAGG97950.083.9901357
CGCTATA48150.083.556714
TATAACG51100.083.4718867
GATATAG86800.083.2892765
Produced by FastQC (version 0.11.5)