FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6036292
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74120.12279061384041726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC75000.088.982336
GGTATAG108350.088.73295
GTATAGG113200.088.327196
GTATACG77150.088.103356
AGTATAC68850.087.5509345
CTATAAC66350.086.909356
CTATACG64750.086.782816
GCTATAC78550.086.777845
GTATACC59600.086.551046
GATATAC65700.086.3983845
CGTATAG68250.086.302255
GGTATAC73800.086.2485055
TTATACG79650.085.994026
TATAGCG100050.085.931077
TTATAGC95150.085.663776
TATAGGC81850.085.423597
CGTATAC73350.085.223415
CCTATAA99750.085.001865
TATAACG74100.084.806747
CCTATAG53650.084.5545045
Produced by FastQC (version 0.11.5)