FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859612
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG61400.2147144437776873No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA52710.18432570572511237No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA43530.15222344849580993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39810.13921469066432787No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT39410.1378158994996524No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA39040.13652201767232758No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA33170.11599475733071479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA1900.092.546532
TTATACG52750.091.486176
GTATAGC36450.091.0885546
CTATACG36350.090.947136
GGTATAG59250.090.750675
CTATAGG40200.090.74436
GTATAGG65450.089.046966
TTATAGC46650.088.481466
CGTATAG38450.088.4521565
CGTATAC30000.088.3496255
TGCGCTA2100.088.258662
TATAGGC49250.088.150377
GCTATAG40200.088.146395
GTATACG55200.088.114036
TCTATAG45700.087.827855
GGTATAA58900.087.338335
GTTATAG61950.087.255485
TATAGGG83750.087.171247
GTTATAC35950.087.071955
GTATAGT53900.087.066756
Produced by FastQC (version 0.11.5)