FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857172
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA82010.1688431045884313No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG80180.16507548013535447No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA79410.16349019552941507No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT61940.12752276427517906No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA53680.11051698395691978No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA52890.10889052312744946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG119500.091.6630255
CTATACG56350.089.941456
CGTATAC49750.089.750645
GTATAGG124800.089.67066
GTATAGC63550.089.5427866
CTATAGG73550.089.315926
TTATACG85650.088.233236
TATAGGG144900.087.9830557
GTATACG90800.087.936916
GGTATAA100600.087.683265
AGTATAG83900.087.472055
CGTATAG74300.087.010585
CCTATAC35450.086.828355
TATACCG72500.086.6775747
TATAGGC89900.086.65017
GTTATAG125350.086.589395
GTATAGT115800.086.549246
GCTATAG81850.086.354915
TATAAGG99450.086.305467
TTATAGC72700.085.982666
Produced by FastQC (version 0.11.5)