FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3810024
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG94940.24918478203811842No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA90070.23640271032413446No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA68100.1787390315651555No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA56170.14742689284896893No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA51400.1349072866732598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46680.12251891326668807No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT41740.10955311567591175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG89850.090.350875
CTATACG49850.089.471766
GTATAGG95500.088.233966
CTATAGG57800.088.177446
GTATAGC50950.088.006226
TTATACG72000.087.8733756
TATAGGC71900.087.731347
AGTATAG62550.087.7128145
GCTATAG59700.087.444345
GTATAGT85200.087.416226
TTATAGC64150.087.371736
AGTATAC41150.086.691895
CGTATAG54350.086.6128855
GGTATAA82250.086.3403245
GTATACG75800.086.099996
CGTATAC38450.085.984725
GATATAC46800.085.86835
TATAGGG114900.085.8622067
GTTATAG97300.085.776295
GCTATAA67400.085.700245
Produced by FastQC (version 0.11.5)