FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844326
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG55060.19357837322444754No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT52100.18317168988364907No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA50430.17730035164745533No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG41710.14664282504888682No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA39280.13809950054951506No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA38260.1345134137226183No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA34760.12220821382640387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG36600.091.623796
CTATAGG43550.090.089976
CCGCGTA1650.089.2782442
TTATACG52750.089.055026
GGTATAG71550.088.9584965
GTATAGG79900.088.815436
CGTATAC27250.088.375875
CGTATAG42750.088.2219545
TATACCG49600.088.198787
AGTATAG49750.088.029875
GCTATAG44550.087.642825
TATAGGG95100.087.3562247
TTATAGC43500.087.136136
GGTATAA57950.087.1308755
GATATAG53850.087.0610055
GGTATAC39700.086.86375
GTATAGT69150.086.755256
TATAGGC49500.086.745687
CCTATAC24250.086.577045
GTATAGC35800.086.373926
Produced by FastQC (version 0.11.5)