FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297317
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG109930.20752014651945505No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA101810.19219163210357243No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA85670.1617233780798846No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA77740.146753535799349No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA61890.11683272871908554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG130700.091.8755955
CTATACG67000.090.247646
CTATAGG71550.088.823676
GTATAGC72250.088.291816
GTATAGG138800.088.187546
TATAGGC95950.087.621477
TTATACG90400.087.483896
GTTATAG134550.086.951965
GGTATAC73300.086.835945
GTATAGT123450.086.6866766
TATAGGG158700.086.557287
CGTATAG74150.086.3535
TCTATAG77400.086.34795
AGTATAG93450.086.00425
TATAGCG130200.085.585337
CGTATAC48350.085.471525
CCTATAG44000.084.961225
TAGCGCG7550.084.931569
TATACCG72450.084.83567
CCTATAC43350.084.810685
Produced by FastQC (version 0.11.5)