FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5882145
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG123260.20954940757155766No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA105970.1801553684922762No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA98270.16706490574441807No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA83410.141802012701149No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA80450.13676983481366067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG156000.090.7999655
CTATACG73350.088.586276
GTATAGG160550.088.547626
CTATAGG87700.088.389576
TTATACG112200.087.758046
GTATAGC84050.087.594026
CGTATAC59750.086.972515
TATAGGG190800.086.956237
CGTATAG86750.086.788545
GTTATAG155350.086.3487555
TATAGGC101000.086.0147257
AGTATAG102500.085.826175
GCTATAG88900.085.811685
GGTATAA124550.085.625
TTATAGC93200.085.517816
TATACCG84050.085.446557
GTATACG122200.085.279746
GTATAGT141200.085.241756
TATAGGT133650.084.548517
GGTATAC90900.084.3939365
Produced by FastQC (version 0.11.5)