FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4231854
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT274780.6493135160144938No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG181160.4280866022315514No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG54150.12795810063390656No Hit
CTCAACTATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGA51380.12141250619704745No Hit
CGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATC50970.12044366369917299No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA48860.11545766938084348No Hit
TTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG45240.10690349903375684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG54050.091.131256
CTATAGG62500.091.0460366
GGTATAG93400.090.9836045
CGTATAC48150.090.857965
GTATACG76000.090.310566
GCTATAC49850.090.141685
CCTATAC35400.090.036575
CTATACC40600.089.967286
TATACCG106700.089.210827
TTATACC51700.089.118016
AGTATAC53650.089.0692755
GGTATAA79250.088.647925
TATAGGG115050.088.30977
GTATAGT95350.088.02386
CCTATAG33000.087.804265
GCTATAG69700.087.572955
AGTATAG66400.087.489925
TATAGGC57450.087.391517
TATACGG84650.087.1983647
TATAAGG72800.087.168457
Produced by FastQC (version 0.11.5)