FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5246398
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT136190.25958762564334614No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG81330.15502064464037993No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG60080.11451666457634362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG129450.090.597765
CTATAGG72800.089.973536
TATAGGG175300.087.762897
CTATACG48200.087.70516
CGTATAC42450.087.280125
TATAGGC74750.087.245137
GTATAGG149000.086.6134346
GTATAGC58250.086.598526
GGTATAA105400.086.483415
TATACGG95550.086.099197
TATAGCG76300.085.970827
TTATACG81150.085.866456
GTATAGT141400.085.6253366
GCTATAG75500.085.5638665
CCTATAG35450.085.487565
TATACCG79400.085.1866157
TATAAGG112100.085.1248257
AGTATAG85750.084.8085255
TATAGGT135950.084.58587
TTATAGC70950.084.553756
Produced by FastQC (version 0.11.5)