FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266605
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT90460.21201868933261925No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG61940.14517397321758166No Hit
GGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATG45380.10636091224755984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG99850.091.57745
CTATAGG57850.090.400176
CTATACG45900.089.9274756
CGTATAC41350.089.029075
GTATAGC52200.087.809626
GTATAGG111800.087.563176
TATAGGG124850.087.38927
TATAGGC63700.087.019537
TATACCG70850.086.6179667
GCTATAG63200.086.4343645
TTATACG65650.086.38836
AGTATAG70850.086.353845
GGTATAC58550.086.240655
TATAGGT89300.086.2217567
TATAGCG79500.085.9165957
CGTATAG64300.085.916075
GGTATAA83650.085.689765
GTTATAG112250.085.565855
TATAACG59750.085.538127
TATAAGG82150.085.1687857
Produced by FastQC (version 0.11.5)