FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5532370
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA129430.23395036846776338No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG92870.16786657436144004No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA74040.13383052832691955No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA63170.11418252936806468No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG63050.1139656241357682No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT60720.10975404754201183No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA59470.10749461803892363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG81100.091.778186
GGTATAG138450.091.5041965
CTATACG61550.090.523496
GTATAGG149200.089.491826
GTATAGC67550.089.303756
GCTATAG84350.088.471235
CGTATAG71050.088.117525
GGTATAA123200.088.0250245
TATAGGG178700.087.900867
TATAGGC86250.087.838537
CGTATAC48350.087.7336045
TTATAGC78750.087.580496
AGTATAG92000.087.413825
CCTATAC34300.087.386875
TTATACG92100.087.263396
AGTATAC54800.086.5089345
GTATAGT132450.085.890466
GTTATAG148300.085.586695
TATAGGT127950.085.271567
TATACCG69300.085.264977
Produced by FastQC (version 0.11.5)