FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5696707
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG127550.090.19695
CTATAGG66950.088.755096
CTATACG43650.087.816446
GTATAGG132350.087.6411746
TATAGGG137700.087.132887
GTATAGC61500.086.966066
CGTATAC49600.086.956515
CCTATAC40300.086.7502445
GCTATAG85950.085.661125
TATAGCG60600.085.513537
CCTATAG39500.085.38065
TATACGG74850.085.2249767
GGTATAA126100.084.792685
CGTATAG69500.084.749055
TATAGGC69100.084.5492557
TATAGGT126300.084.542617
GATATAG101250.084.5391165
AGTATAG90000.084.128625
CTATACC42950.084.049846
TTATACG74350.084.010066
Produced by FastQC (version 0.11.5)