FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4486922
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA65920.1469158590231789No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA50990.11364137820982848No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT49840.11107837399446659No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG49670.11069949511045656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG102850.090.566695
CTATACG47350.089.782856
GTATAGG110100.089.175086
TTATACG73550.088.67036
CTATAGG60700.088.5825356
CCTATAC31300.088.474775
TATAGGG131400.088.274617
GTATAGC53600.087.909786
CGTATAG55750.087.6729665
TATAGGC71550.087.363857
CGTATAC40250.087.2114945
GCTATAG66800.086.965145
TTATAGC70150.086.1967856
GGTATAA98150.086.192365
AGTATAG77550.086.118955
TATAGCG90400.085.750627
TATACGG101450.085.21287
CCTATAG36000.084.972535
GTATAGT101800.084.967446
CGCTATA46600.084.7052464
Produced by FastQC (version 0.11.5)